![]() oneidensis, producing an oxidative current upon the addition of 1.5 mM glucose. Artificial biofilm was also tested with Pseudomonas fluorescens instead of S. The current at the anode was 10 and 4 times higher with the artificial biofilm (0.027 A m2) than with film composed with bacteria alone (0.003 A m2) or associated with cytochrome c (0.007 A m2). Bacterial viability was studied and metabolic activity was characterized with the help of electrochemistry. These analyzes showed the importance of the cytochrome c in the assembly and organization of the biocomposite. The self-assembly was first studied by diffusion light scattering, scanning electron microscopy and Raman spectroscopy. ![]() ![]() A biocomposite material was proposed from the self-assembly of the bacteria Shewanella oneidensis with carbon nanotubes and cytochrome c (extract from bovine heart). The aim of this PhD work was to design an artificial electroactive biofilm in order to optimize extracellular electron transfers (EET) by artificially reconstituting the biofilm in the presence of exogenous materials. ![]()
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